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Curr Biol:英国学者发现食肉菌可能对抗超级病菌

摘要 : 2016年11月17日,国际著名学术杂志《Cell》子刊《Current Biology》杂志在线发表了英国伦敦帝国理工学院Serge Mostowy研究员的一篇研究论文,研究介绍了一种自然界存在的食肉性细菌能与免疫系统合作清除斑马鱼体内多重耐药菌志贺氏杆菌。

 2016年11月17日,国际著名学术杂志《Cell》子刊《Current Biology》杂志在线发表了英国伦敦帝国理工学院Serge Mostowy研究员的一篇研究论文,研究介绍了一种自然界存在的食肉性细菌能与免疫系统合作清除斑马鱼体内多重耐药菌志贺氏杆菌。这是噬菌蛭弧菌首次成功用于抗菌治疗,标志着向战胜耐药菌或超级细菌迈出了重要一步。

志贺氏杆菌感染能引发腹泻,每年造成1.6亿病例,其中超过100万人死亡。而且,该病菌的耐药性愈加严重。于是,英国伦敦帝国理工学院和诺丁汉大学的研究团队,决定用蛭弧菌对抗耐药的志贺氏杆菌。当研究人员将这两种细菌放在实验室一起培养时,蛭弧菌让志贺氏杆菌的数量下降了数千倍。为了验证蛭弧菌控制耐药的革兰氏阴性感染的能力,他们用志贺氏杆菌M90T感染斑马鱼幼体,同时又让其感染蛭弧菌。

结果斑马鱼幼体的成活率达到60%。而在对照组中,没有感染蛭弧菌的斑马鱼幼体,在感染的第三天,成活率仅有25%。Serge Mostowy表示,“这项研究的确让人们看到了蛭弧菌的独特与奇妙之处,它展现了一种与免疫系统浑然天成的协同作用,如果该菌在人体内存在的时间足够长,那它就能在细菌被自然清除前干掉它们。”

诺丁汉大学Liz Sockett指出,蛭弧菌的侵略行为破坏了志贺氏杆菌细胞,并且刺激了白细胞,由于它们对志贺氏杆菌的加倍作用,最终导致斑马鱼幼体成活率的提高。不过,研究人员还表示,食肉菌与宿主白细胞之间似乎也出现了“恶战”,最终免疫力强的斑马鱼活了下来。

实际上,蛭弧菌在自然界中能杀死大肠杆菌和沙门氏菌等多种革兰氏阴性菌。也有研究发现,它能减少鸡胃部的病菌。当然,蛭弧菌能否在人体中取得成功尚不得而知。但研究人员表示,这是一种积极信号,也正是人们与耐药性危机战斗的关键。

 

原文链接:

Injections of Predatory Bacteria Work Alongside Host Immune Cells to Treat Shigella Infection in Zebrafish Larvae

原文摘要:

Bdellovibrio bacteriovorus are predatory bacteria that invade and kill a range of Gram-negative bacterial pathogens in natural environments and in vitro [ 1, 2 ]. In this study, we investigated Bdellovibrio as an injected, antibacterial treatment in vivo, using zebrafish (Danio rerio) larvae infected with an antibiotic-resistant strain of the human pathogen Shigella flexneri. When injected alone, Bdellovibrio can persist for more than 24 hr in vivo yet exert no pathogenic effects on zebrafish larvae. Bdellovibrio injection of zebrafish containing a lethal dose of Shigella promotes pathogen killing, leading to increased zebrafish survival. Live-cell imaging of infected zebrafish reveals that Shigella undergo rounding induced by the invasive predation from Bdellovibrio in vivo. Furthermore, Shigella-dependent replication ofBdellovibrio was captured inside the zebrafish larvae, indicating active predation in vivo. Bdellovibrio can be engulfed and ultimately eliminated by host neutrophils and macrophages, yet have a sufficient dwell time to prey on pathogens. Experiments in immune-compromised zebrafish reveal that maximal therapeutic benefits of Bdellovibrio result from the synergy of both bacterial predation and host immunity, but that in vivo predation contributes significantly to the survival outcome. Our results demonstrate that successful antibacterial therapy can be achieved via the host immune system working together with bacterial predation by Bdellovibrio. Such cooperation may be important to consider in the fight against antibiotic-resistant infections in vivo.

来源: Current Biology 浏览次数:0

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