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Curr Biol:农科院植保所王桂荣研究团队揭示棉铃虫交配调控作用新机制

摘要 : 2017年5月18日, 国际著名学术杂志《Cell》子刊《Current Biology》杂志在线发表了中国农业科学院植物保护研究所王桂荣研究团队题为“A pheromone antagonist regulates optimal mating time in the moth, Helicoverpa amigera”的研究论文

 2017年5月18日, 国际著名学术杂志《Cell》子刊《Current Biology》杂志在线发表了中国农业科学院植物保护研究所王桂荣研究团队题为“A pheromone antagonist regulates optimal mating time in the moth, Helicoverpa amigera”的研究论文,研究利用最新的CRISPR/Cas9基因编辑技术结合神经电生理记录等技术,首次揭示了性信息素拮抗剂参与调控棉铃虫最优交配时间并阐明了其作用的分子机制。博士研究生常贺坦和刘杨副研究员是文章的共同第一作者,王桂荣研究员为论文通讯作者。

化学通讯在昆虫寻找配偶、食物以及产卵地点等过程中起着非常重要的作用,鳞翅目昆虫尤其是蛾类依靠特异的性信息素通讯来寻找和识别适合交配的同种配偶。棉铃虫雌虫性腺释放多种性信息素组分,其中性信息素主组分具有吸引雄虫进行交配的功能,但是性信息素次要组分功能不清楚,推测可能在与同域近缘种形成生殖隔离中起作用。此项研究首先发现棉铃虫雌蛾挥发性信息素主成分的时间与交配高峰期不一致,进一步的研究发现不同日龄雌虫腺体中Z11-16:OH的含量与棉铃虫交配率成负相关,由此推测“性信息素次要组分Z11-16:OH可能参与调控棉铃虫的交配”。利用CRISPR/Cas9敲除棉铃虫感受Z11-16:OH的受体OR16,突变体雄虫丧失了对Z11-16:OH的电生理反应以及驱避行为反应。更重要的是,突变体雄虫不能区分性成熟和未成熟的雌虫,在性信息素主成分的作用下与未成熟的雌虫进行交配,使子代的孵化率和存活率显著降低。

该研究首次报道了性信息素次要组分作为拮抗剂,与性信息素主组分一起参与调控害虫最优交配时间,并阐明了这种调控作用的分子机制。该研究为基于性信息素拮抗剂发展害虫驱避剂和交配干扰剂提供了新的思路。

原文链接:

A Pheromone Antagonist Regulates Optimal Mating Time in the MothHelicoverpa armigera

原文摘要:

Many insect species use multi-component sex pheromones to discriminate among potential mating partners [ 1–5 ]. In moths, pheromone blends tend to be dominated by one or two major components, but behavioral responses are frequently optimized by the inclusion of less abundant minor components [ 6 ]. An increasing number of studies have shown that female insects use these chemicals to convey their mating availability to males, who can assess the maturity of females and thus decide when to mate [ 7, 8 ]. However, little is known about the biological mechanisms that enable males to assess female reproductive status. In this study, we found that females ofHelicoverpa armigera avoid nonoptimal mating by inhibiting males with pheromone antagonist cis-11-Hexadecenol (Z11-16:OH). We also show that this antagonist-mediated optimization of mating time ensures maximum fecundity. To further investigate molecular aspects of this phenomenon, we used the CRISPR/Cas9 system to knock out odorant receptor 16 (OR16), the only pheromone receptor tuned to Z11-16:OH. In mutant males, electrophysiological and behavioral responses to Z11-16:OH were abolished. Inability to detect Z11-16:OH prompted the males to mate with immature females, which resulted in significantly reduced viability of eggs. In conclusion, our study demonstrates that the sensitivity of OR16 to Z11-16:OH regulates optimal mating time and thus ensures maximum fecundity. These results may suggest novel strategies to disrupt pest insect mating.

来源: Current Biology 浏览次数:0

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