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Mol Plant:中科院版纳植物园所余求迪研究组揭示拟南芥WRKY转录因子调控植物开花分子机理

摘要 : 近日,国际著名学术杂志《Cell》子刊《Molecular Plant》杂志在线发表了中国科学院西双版纳热带植物园余迪求研究员题为“The Arabidopsis WRKY transcription factors WRKY12 and WRKY13 oppositely regulate flowering under short-day conditions”的研究论文

 近日,国际著名学术杂志《Cell》子刊《Molecular Plant》杂志在线发表了中国科学院西双版纳热带植物园余迪求研究员题为“The Arabidopsis WRKY transcription factors WRKY12 and WRKY13 oppositely regulate flowering under short-day conditions”的研究论文,博士研究生李委、王后平为该论文的共同第一作者,余迪求研究员为该论文的通讯作者。

WRKY转录因子是植物特有的一类蛋白质,在模式植物拟南芥中一共有74个成员,其主要功能是参与调控植物逆境胁迫反应。目前的研究表明,除了调控植物逆境胁迫外,WRKY转录因子还参与调控植物发育过程;然而,WRKY转录因子是否参与调控植物开花诱导及其信号网络和分子机理?目前还没有相关报道。

余迪求研究团队研究发现,拟南芥WRKY12和WRKY13分别正调控和负调控植物在短日照条件下的开花, WRKY12基因突变导致植物开花延迟,而WRKY13基因突变使得植物开花提前。分子生物学及生物化学证据表明,WRKY12及WRKY13能够结合到花诱导信号基因FUL基因的启动子区域,进而直接调控FUL基因的表达。进一步研究表明,植物激素赤霉素(GA)信号途径的关键抑制子DELLA蛋白能有效地与WRKY12和WRKY13相互作用形成蛋白复合物,并影响它们的转录激活功能。该研究证实WRKY12和WRKY13功能通过相互拮抗的方式参与调控赤霉素介导的植物开花诱导。

原文链接:

The Arabidopsis WRKY transcription factors WRKY12 and WRKY13 oppositely regulate flowering under short-day conditions

原文摘要:

In plants, photoperiod is an important cue for determining flowering. An earlier floral transition is observed in Arabidopsis thaliana under long-day (LD) than under short-day (SD) conditions, with flowering under the latter being mainly regulated by the plant hormone gibberellin (GA). Here, we report two WRKY proteins that oppositely function in controlling flowering time under short-day conditions. Phenotypic analysis showed that disruption of WRKY12 caused a delay in flowering, while disruption of WRKY13 induced flowering. Promoter-swap experiments displayed the negatively correlated expression profiles of these proteins during the regulation of flowering time. Molecular and genetic analysis demonstrated that the floral inductive signal FRUITFULL (FUL) as a direct downstream target gene of WRKY12 and WRKY13. Through yeast two-hybrid screening, the DELLA proteins GIBBERELLIN INSENSITIVE (GAI) and RGA-LIKE1 (RGL1) were identified as interacting with WRKY12 and WRKY13. The interactions between DELLAs and the WRKYs interfered with the transcriptional activity of the WRKY proteins. Moreover, WRKY12 and WRKY13 partly mediated the effect of GA3on the control of flowering time. Taken together, our study indicates that WRKY12 and WRKY13 oppositely modulate flowering time under SD conditions.

来源: Molecular Plant 浏览次数:0

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