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Mol Plant:中科院上海生科院方玉达研究组揭示植物细胞核纤层类似蛋白调控植物免疫新功能

摘要 : 2017年8月18日,国际著名学术杂志《Cell》子刊《Molecular Plant》杂志在线发表了中国科学院上海生命科学研究院上海植物生理生态研究所方玉达研究组的一篇研究论文

2017年8月18日,国际著名学术杂志《Cell》子刊《Molecular Plant》杂志在线发表了中国科学院上海生命科学研究院上海植物生理生态研究所方玉达研究组的一篇研究论文,论文题为Lamin-like proteins negatively regulate plant immunity through NAC WITH TRANSMEMBRANE MOTIF1-LIKE9 and NonEXPRESSOR OF PR GENES1 in Arabidopsis thaliana,文章揭示了拟南芥核纤层类似蛋白负调控植物免疫过程。

核纤层蛋白在动物细胞中参与众多细胞核内重要的生物学过程,CROWDED NUCLEI (CRWN)家族蛋白作为拟南芥中核纤层类似蛋白,其功能却知之甚少。方玉达研究组发现,crwn1 crwn2双突变体对病原菌具有较强的抗性。CRWN 1基因在转录和转录后水平都受到了病原菌及水杨酸的调控。CRWN 1通过抗病途径的NAC类转录因子NTL9相互作用,加强NTL9对下游抗病基因PR1的转录抑制。进一步的遗传实验表明,crwn1 crwn2的抗病相关表型依赖于抗病通路中的关键蛋白NPR1。方玉达研究组的研究发现了由CRWN1、NTL9 及NPR1等蛋白组成的调控PR1基因表达的分子遗传网络。

植物细胞核纤层类似蛋白调控植物免疫新功能

原文链接:

Lamin-like Proteins Negatively Regulate Plant Immunity through NAC WITH TRANSMEMBRANE MOTIF1-LIKE9 and NonEXPRESSOR OF PR GENES1 in Arabidopsis thaliana.

原文摘要:

Nuclear lamins are involved in multiple biological processes in metazoan cells. The proteins of the CROWDED NUCLEI (CRWN) family are considered lamin-like candidates in Arabidopsis, although the functions of these proteins are largely unknown. In this article we show that crwn1 crwn2 double mutant displays an enhanced resistance against virulent bacterial pathogens, and both virulent bacteria and salicylic acid (SA) induce transcription of CRWN1 gene as well as proteasome-mediated degradation of CRWN1 protein. We also show that CRWN1 interacts with NAC WITH TRANSMEMBRANE MOTIF1-LIKE9 (NTL9), a NAC transcription factor involved in plant immunity. The interaction between CRWN1 and NTL9 enhances the binding of NTL9 to the promoter of the PATHOGENESIS-RELATED1 (PR1) gene, and inhibits PR1 expression. Further genetic experiments indicated that the defense-related phenotypes of crwn1 crwn2 double mutant are dependent on NonEXPRESSOR OF PR GENES1 (NPR1), a transcriptional cofactor of PR1. These findings revealed a regulatory network composed of lamin-like protein CRWN1, NTL9, and NPR1 for the regulation of PR1 expression.

DOI: 10.1016/j.molp.2017.09.008

来源: Molecular Plant 浏览次数:0

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