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Stem Cell Reports:武汉大学周荣家和程汉华教授研究组发表干细胞分化研究新成果

摘要 : 2016年9月13日,国际著名学术杂志《Cell》子刊《Stem Cell Reports》杂志在线发表了武汉大学生命科学学院周荣家教授和程汉华教授实验室在干细胞及其分化研究新成果

 2016年9月13日,国际著名学术杂志《Cell》子刊《Stem Cell Reports》杂志在线发表了武汉大学生命科学学院周荣家教授和程汉华教授实验室在干细胞及其分化研究新成果,研究成果为:Directed differentiation of zebrafish pluripotent embryonic cells to functional cardiomyocytes。项研究通过确立斑马鱼胚胎细胞的多能性时序,建立了直接诱导为功能性心肌细胞的技术系统。博士后肖遥同学为论文第一作者,周荣家教授和程汉华教授为论文共同通讯作者。

胚胎干细胞具有发育的全能性及自我更新能力,可以分化为多样性的功能细胞,参与个体发育和生命的延续。该项研究通过分子及发育生物学等研究手段,确定了斑马鱼胚胎细胞的多能性时序,发现其胚胎干性维持在基因组激活后一个狭窄的时间窗口。随着胚胎发育进程,进而进行内胚层、中胚层和外胚层等三胚层的分化。基于胚胎细胞干性窗口,可以直接诱导分化。研究人员通过直接培养诱导斑马鱼胚胎细胞,成功建立了功能性心肌细胞诱导系统。分化的心肌细胞表现出正常的收缩动力学和电生理学特性。该项成果在细胞发育与分化、药物筛选、细胞治疗和疾病模型等研究中有着广阔的应用前景。

图:斑马鱼胚胎干细胞时序及其功能性心肌细胞的诱导

原文链接:

Directed Differentiation of Zebrafish Pluripotent Embryonic Cells to Functional Cardiomyocytes

原文摘要:

A cardiomyocyte differentiation in vitro system from zebrafish embryos remains to be established. Here, we have determined pluripotency window of zebrafish embryos by analyzing their gene-expression patterns of pluripotency factors together with markers of three germ layers, and have found that zebrafish undergoes a very narrow period of pluripotency maintenance from zygotic genome activation to a brief moment after oblong stage. based on the pluripotency and a combination of appropriate conditions, we established a rapid and efficient method for cardiomyocyte generation in vitro from primary embryonic cells. The induced cardiomyocytes differentiated into functional and specific cardiomyocyte subtypes. Notably, these in vitro generated cardiomyocytes exhibited typical contractile kinetics and electrophysiological features. The system provides a new paradigm of cardiomyocyte differentiation from primary embryonic cells in zebrafish. The technology provides a new platform for the study of heart development and regeneration, in addition to drug discovery, disease modeling, and assessment of cardiotoxic agents.

来源: Stem Cell Reports 浏览次数:0

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